Using CRISPR To Treat Cystic Fibrosis

Using CRISPR To Treat Cystic Fibrosis

In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.

Group Size:
For 8 Gels

Time Required:
Complete in 45 minutes

Kit Includes:
Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue™ DNA Stain.

All You Need:
DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.

Storage:
Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended.