Nutrient Agar - 100g

Nutrient Agar - 100g

Powdered nutrient agar suitable for preparing culture plates or solutions for the growth of bacterial or fungal cultures.

Question: Having made my agar powder into solution it will not melt again at 50°C, as required for a practical. How do I re-melt it?

Answer: Agar typically solidifies at around 30-40°C, but it can remain firm at temperatures as high as 65°C or so and will often not melt again fully until the temperature reaches 85°C to 95°C (depending on type). This property is known as hysteresis.
You need to bring the solidified agar up to 85-95°C  by standing the bottle in an appropriate water bath, and then allow it cool to 50°C, maintaining it at 50°C in a second water bath, at which temperature it should remain liquid. 

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