Construction and Cloning of a DNA Recombinant

Edvotek Kit: Construction and Cloning of a DNA Recombinant

Cloning is frequently performed to study gene structure and function, and to enhance gene expression. This experiment is divided into five modules:

• Clones are constructed by ligation of a vector and a fragment insert.
• The constructs are then transformed into competent cells and the cells are grown and selected for resistance.
• Plasmid DNA is then isolated from the transformants, cleaved with restriction enzymes, and analyzed by agarose gel electrophoresis.

Key Features:

• Enough kit for 5 plasmid constructs & analyses
• Complete 5 modules in approximately 5 hours
• Recommended for college-level courses

Some Components Require Freezer & Refrigerator Storage

See specification for more details

Kit Includes:

• Instructions, BactoBeads™,
• enzymes,
• plasmid DNA,
• restriction enzyme dilution buffer,
• enzyme grade water,
• standard DNA fragments,
• restriction enzyme reaction buffer,
• gel loading solution,
• agarose powder,
• electrophoresis buffer,
• SYBR® Safe DNA stain,
• calibrated pipet

You Need:

• Electrophoresis apparatus and power supply,
• automatic micropipette with tips,
• balance, microwave or hot plate,
• water bath,
• large weigh boats for staining,
• UV transilluminator or blue light visualization system,
• floating racks for microtest tubes,
• pipet pump or bulb,
• 5 or 10 ml pipets,
• laboratory glassware,
• metric rulers,
• distilled water,
• ice